Journal: Antibiotics

Article Title: New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo -CBP 3 -PepI against Klebsiella pneumoniae

doi: 10.3390/antibiotics11121753

Figure Lengend Snippet: Cell viability and antibiofilm potential of Mo -CBP3-PepI against K. pneumoniae .

Article Snippet: Cytadherence high molecular weight protein 2 , P75471 , Mycoplasma pneumoniae (strain ATCC 29342/M129) , Cytoplasm , 1.682.

Techniques: Inhibition

Journal: Antibiotics

Article Title: New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo -CBP 3 -PepI against Klebsiella pneumoniae

doi: 10.3390/antibiotics11121753

Figure Lengend Snippet: Mechanism of action of Mo -CBP3-PepI assessed by fluorescence microscopy. In all experiments, Mo -CBP3-PepI was used at a concentration of 31.26 μg mL −1 . ( A , B ) control K. pneumoniae cells for PI uptake assay. ( E , F ) Mo -CBP3-PepI-treated K. pneumoniae cells for PI uptake assay. Red fluorescence ( F ) indicates pore formation induced by Mo -CBP3-PepI. ( C , D ) control K. pneumoniae cells for FITC-Dextran uptake assay. ( G , H ) Mo -CBP3-PepI-treated K. pneumoniae cells for FITC-Dextran uptake assay. Green fluorescence ( H ) indicates 10-kDa -sized pore formation induced by Mo -CBP3-PepI. ( I , J ) control K. pneumoniae cells for ROS overproduction assay. ( K , L ) Mo -CBP3-PepI-treated K. pneumoniae cells for ROS overproduction assay. Green fluorescence ( L ) indicates pore formation induced by Mo -CBP3-PepI. Bars indicate 100 μm.

Article Snippet: Cytadherence high molecular weight protein 2 , P75471 , Mycoplasma pneumoniae (strain ATCC 29342/M129) , Cytoplasm , 1.682.

Techniques: Fluorescence, Microscopy, Concentration Assay, Control

Journal: Antibiotics

Article Title: New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo -CBP 3 -PepI against Klebsiella pneumoniae

doi: 10.3390/antibiotics11121753

Figure Lengend Snippet: Scanning electron microscope of K. pneumoniae cells treated with Mo -CBP3-PepI. ( A ) control K. pneumonia cells treated with DMSO-NaCl solution. ( B – H ) K. pneumoniae cells treated with Mo -CBP3-PepI 31.26 μg mL −1 .

Article Snippet: Cytadherence high molecular weight protein 2 , P75471 , Mycoplasma pneumoniae (strain ATCC 29342/M129) , Cytoplasm , 1.682.

Techniques: Microscopy, Control

Journal: Antibiotics

Article Title: New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo -CBP 3 -PepI against Klebsiella pneumoniae

doi: 10.3390/antibiotics11121753

Figure Lengend Snippet: Distribution of K. pneumoniae proteins obtained after treatment with Mo -CBP3-PepI. ( A ) Venn diagram shows the dispersion of total proteins of control and Mo -CBP3-PepI-treated cells. ( B ) Represents the fold-change value of overlapping proteins, which were found in both groups. The vertical bar indicates the color scale according to each protein’s fold-change value.

Article Snippet: Cytadherence high molecular weight protein 2 , P75471 , Mycoplasma pneumoniae (strain ATCC 29342/M129) , Cytoplasm , 1.682.

Techniques: Dispersion, Control

Journal: Antibiotics

Article Title: New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo -CBP 3 -PepI against Klebsiella pneumoniae

doi: 10.3390/antibiotics11121753

Figure Lengend Snippet: Classification proteins from K. pneumoniae cells identified by LC-ESI-MS/MS analysis. The overlapping proteins (found in both control and treated) were classified based on biological process and molecular function.

Article Snippet: Cytadherence high molecular weight protein 2 , P75471 , Mycoplasma pneumoniae (strain ATCC 29342/M129) , Cytoplasm , 1.682.

Techniques: Tandem Mass Spectroscopy, Control

Journal: Antibiotics

Article Title: New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo -CBP 3 -PepI against Klebsiella pneumoniae

doi: 10.3390/antibiotics11121753

Figure Lengend Snippet: Differentially accumulated proteins identified by ESI-LC-MS/MS.

Article Snippet: Cytadherence high molecular weight protein 2 , P75471 , Mycoplasma pneumoniae (strain ATCC 29342/M129) , Cytoplasm , 1.682.

Techniques: Clinical Proteomics, Membrane, Modification, High Molecular Weight

Journal: Antibiotics

Article Title: New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo -CBP 3 -PepI against Klebsiella pneumoniae

doi: 10.3390/antibiotics11121753

Figure Lengend Snippet: Scheme showing the mechanisms of antibacterial action of Mo -CBP3-PepI against K. pneumoniae . This scheme was produced only with proteins that decreased in abundance. The numbers explain important process. All them are explained in the text.

Article Snippet: Cytadherence high molecular weight protein 2 , P75471 , Mycoplasma pneumoniae (strain ATCC 29342/M129) , Cytoplasm , 1.682.

Techniques: Produced

Journal:

Article Title: Interferon (IFN)-? Gene Transfer into TS/A Adenocarcinoma Cells and Comparison with IFN-?

doi:

Figure Lengend Snippet: In Vitro Production of IFN-β by Transduced TS/A Clones

Article Snippet: The LMuIFN-βSN retroviral vector was constructed by insertion of a 680-bp murine IFN-β Pst I complementary DNA fragment into the unique Pst I site of the pSP72 plasmid (Promega, Madison, WI).

Techniques: In Vitro, Plasmid Preparation